Sugarcane bagasse is a potential feedstock for cellulosic ethanol production, rich in both glucan and xylan. This stresses the importance of utilizing both C6ÿand C5ÿsugars for conversion into ethanol in order to improve the process economics. During processing of the hydrolysate degradation products such as acetate, 5-hydroxymethylfurfural (HMF) and furfural are formed, which are known to inhibit microbial growth at higher concentrations. In the current study, conversion of both glucose and xylose sugars into ethanol in wet exploded bagasse hydrolysates was investigated without detoxification usingÿScheffersomyces (Pichia) stipitisÿCBS6054, a native xylose utilizing yeast strain. The sugar utilization ratio and ethanol yield (Yp/s) ranged from 88-100% and 0.33-0.41?ñ?0.02ÿg/g, respectively, in all the hydrolysates tested. Hydrolysate after wet explosion at 185øC and 6ÿbar O2, composed of mixed sugars (glucose and xylose) and inhibitors such as acetate, HMF and furfural at concentrations of 3.2?ñ?0.1, 0.4 and 0.5ÿg/l, respectively, exhibited highest cell growth rate of 0.079ÿg/l/h and an ethanol yield of 0.39?ñ?0.02ÿg/g sugar converted.ÿScheffersomyces stipitisÿexhibited prolonged fermentation time on bagasse hydrolysate after wet explosion at 200øC and 6ÿbar O2ÿwhere the inhibitors concentration was further increased. Nonetheless, ethanol was produced up to 18.7?ñ?1.1ÿg/l resulting in a yield of 0.38?ñ?0.02ÿg/g after 82ÿh of fermentation.

Author Name: Rajib Biswas, Hinrich Uellendahl, Birgitte K Ahring

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Keywords: 21910855,Conversion of C6ÿand C5ÿsugars in undetoxified wet exploded bagasse hydrolysates usingÿScheffersomyces(Pichia) stipitisÿCBS6054,21910855,Rajib Biswas, Hinrich Uellendahl, Birgitte K Ahring

ISSN: 21910855

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