Tissue engineering encapsulated chondrocytes in carrier matrix have been
considered to be a promising technique for regeneration of cartilage. However,
cell expansion is always accompanied by lost of chondrocyte phenotype and
cellular dedifferentiation. Sticophus chloronotus, a marine sea cucumber
invertebrate is rich in n-3 PUFAs and phenolic compound, which can help in the
regeneration of the cartilage. In this study, we evaluated the effect of sticophus
chloronotus aqueous extract on human osteoarthritis chondrocytes (HOC) invitro.
The HOC isolated from the knee joint cartilage were culture in different
concentrations of sticophus chloronotus aqueous extract (SCAE). The cultured
chondrocytes were evaluated by means cell morphology and proliferation,
quantitative phenotypic expression and immunochemistry technique of collagen
type 1, 11, aggrecan core protein and sox-9. Monolayer chondrocytes were
stained with toluidine blue and sGAG production was analyzed after 7 days in
culture.The HOC cultured in various SCAE appeared an almost polygonal
morphology maintaining their chondrocytes characteristic. SCAE
supplementation promoted chondrocytes proliferation, upregulated the expression
of cartilage specific markers and increased immunopositivity of collagen type 11,
aggrecan core protein and sox-9 as compared to control. HOC showed higher
sulfated glycosaminoglycan (sGAG) accumulation, concomitant with significant
toluidine blue staining in culture supplemented with SCAE. Supplementation of
SCAE could effectively promote chondrocyte growth, enhanced secretion and
synthesis of cartilage extracellular matrix. This study showed that sticophus
chloronotus may be useful as a pro-chondrogenic agent, and may be potentially
useful as a therapeutic agent for osteoarthritis
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Author Name: Mohd Heikal MY*, Ahmad Nazrun S, Mohd Fauzi Busra, Chua KH, Norzana AG, Rizal AR
URL: View PDF
Keywords: Sticophus chloronotus; Human osteoarthritis articular chondrocytes; prochondrogenic agent; Dedifferentiation
ISSN: 2349-5340
EISSN: 2349-5340
EOI/DOI: 10.5281/zenodo.851910
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