The refinement of biodiesel or renewable diesel from bacterial lipids has a great potential to make a contribution for energy production in the future. This study provides new data concerning suitable nutrient concentrations for cultivation of the Gram-positiveÿRhodococcus opacusÿPD630, which is able to accumulate large amounts of lipids during nitrogen limitation. Enhanced concentrations of magnesium have been shown to increase the final optical density and the lipid content of the cells. Elevated phosphate concentrations slowed down the onset of the accumulation phase, without a clear effect on the final optical density and the cell?s lipid content. A robust growth ofÿR. opacusÿwas possible in the presence of ammonium concentrations of up to 1.4ÿgÿl-1ÿand sucrose concentrations of up to 240ÿgÿl-1, with an optimum regarding growth and lipid storage observed in the range of 0.2 to 0.4ÿgÿl-1ÿammonium and 20 to 40ÿgÿl-1ÿsucrose, respectively. Moreover,ÿR. opacusÿshowed tolerance to high salt concentrations.
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Author Name: Helge Janáen, Mohammad H A Ibrahim, Daniel Br”ker, Alexander Steinbchel
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Keywords: 21910855,Optimization of macroelement concentrations, pH and osmolarity for triacylglycerol accumulation inRhodococcus opacusÿstrain PD630,21910855,Helge Janáen, Mohammad H A Ibrahim, Daniel Br”ker, Alexander Steinbchel
ISSN: 21910855
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