A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Methoxsalen in tablet dosage form. An Inertsil ODS C-18, 5?m column having 250 x 4.6mm internal diameter in isocratic mode with mobile phase containing Acetonitrile: Tetra hydro furon: 0.01M NaH2Po4 in the ratio of 65:15:25 (v/v/v) was used. The flow rate was 1.0ml/min and effluents were monitored at 220nm. The retention time for Methoxsalen was 4.447min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection and limit of quantification were found to be 0.04ppm and 0.13ppm respectively and recovery of Methoxsalen from tablet formulation was found to be 98.23%. The proposed method was successfully applied for the quantitative determination of Methoxsalen in tablet formulation
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Author Name: CHILUKURI DURGA JYOTHI*, K.LAKSHMI NARAYANA, M.MALATHI LATHA, B.MADHAVI and K.RAMBABU
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Keywords: Methoxsalen, HPLC, Linearity, Validation, Robustness.
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EISSN: 2231- 0541
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