The study was an attempt to study the cellular proliferation, by a means of Ki-67 labeling index, in oral mucosal intraepithelial lesions and squamous cell carcinoma to evaluate its potential association with their histologic grades. The cellular proliferation was also evaluated on sections taken from hyperplastic and normal mucosal epithelium in order to determine whether it can be used as a diagnostic aid in evaluating oral mucosal atypia. Fifty five patients with oral squamous cell carcinoma (n=19), intraepithelial lesions (n=16), hyperplastic epithelium (n=13) and normal mucosal epithelium (n=7) were enrolled in the study. The Ki-67 labeling index was determined by immunohistochemistry on paraffin sections using avidin-biotin technique and antigen retrieval was done by a pressure pot. Overall, high Ki-67 labeling index was observed in 52.6% of squamous cell carcinoma cases, 62.5% of intraepithelial lesions, 23.1% of hyperplastic epithelium, and nearly none in benign epithelium. The high index was significantly high in both carcinoma and intraepithelial lesions compared with that of hyperplastic epithelium and the high index was associated with graded both carcinoma and intraepithelial lesions. This study confirms and extends previous findings that the Ki-67 labeling index can be used as an indicator to predict the oral mucosal atypia as a pre-malignant or malignant lesion. Its immunohistochemistry emerges as a clinically useful supplement for histopathological assessment of severity of oral squamous cell carcinoma and intraepithelial lesions.
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Author Name: Intisar Salim Pity, Sabria Najeeb Ibrahim
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Keywords: IJSER,2229-5518,22295518,Intisar Salim Pity, Sabria Najeeb Ibrahim