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CYCLOOXYGENASE INHIBITORY, ANTI-INFLAMMATORY AND ANTI-OXIDANT EFFECTS OF WRIGHTIA TINCTORIA R.BR LEAVES

Polyphenol contents, antioxidant and anti-inflammatory activities of Wrightia tinctoria (Roxb.) R.Br. leaves were investigated in this study in order to provide a scientific basis for the traditional use of plants to treat chronic inflammatory skin diseases such as psoriasis. The total phenolic content and flavonoid content was accessed using Folin-ciocalteu and AlCl3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and nitric oxide scavenging methods. The anti-inflammatory activity was evaluated using carageenan induced paw edema, cotton pellet granuloma pouch method in rats, membrane stabilizing potential and cyclooxygenase inhibition assay. The total phenolic content and flavonoid content was found to be 10.80% w/w and 1.7% w/w respectively. The HPTLC densitometric estimation of rutin was found to be 0.254 mg/gm. The ethanolic extract of Wrightia tinctoria with a concentration of 400 mg/kg b.w. showed maximum (56.25%) inhibition on carrageenan induced rat paw edema, significantly inhibited granuloma formation (p<0.01) in cotton pellet granuloma model. The ethanol extract (at concentration range of 0.10-0.40 mg/ml) significantly protect the rat erythrocyte membrane against lysis induced by hypotonic solution. The ethanol extract was found to be a potent COX-2 inhibitor with an IC50 value of 64 µg/ml and a weak inhibitory effects on COX-1. The tested extract showed good dose-dependent free radical scavenging property in all the models. The IC50 values were found to be 150 attributed to the presence of flavonoids and other polyphenols in the extract. These experimental results were 160 µg/ml and 80 µg/ml respectively. The anti-inflammatory activity of W. tinctoria leaves could be at least in part due to COX-2 enzyme inhibition and free radical-scavenging activities which may behave established a pharmacological evidence for the folklore claim of the drug to be used as an anti inflammatory agent.



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Keywords: COX-1/COX-2, Flavonoid, Inflammation, Polyphenol, Rutin

ISSN: 2321–0494

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