Diabetes mellitus is considered as a non-infectious epidemic of the 21st century. This pathology is estimated by the WHO to affect about 347 million of people all over the world. One of the most frequent complications of diabetes is cerebral ischemia disturbing energy properties of the neurons and glia resulting in intense generation of free radical compounds, disturbance of the membrane integrity, and becomes a cause of cellular death. Literary review on the problem investigated has not found the answer concerning the peculiarities of nucleic hemostasis disorders under conditions of a combined influence of ischemia-reperfusion and diabetes mellitus in different cortical structures which is indicative of the importance of the given work.
The objective of our research was to study dynamic changes of RNA content in the
nervous and glial cells in the parietal, frontal and temporal cortical lobes of the cerebral hemispheres in rats with diabetes mellitus complicated by ischemic-reperfusion lesion.
Research was conducted on 66 white male rats of nonlinear control group and with
streptozotocin-induced diabetes. The latter was simulated by single traperitoneal introduction of streptozotocin to two-month old animals in a dose of 60 mg/kg. In some rats in the control group and those who had three diabetes, carotid double headed simulated ischemia, which under intraperitoneal anesthesia anterior median cervical access isolated both common carotid arteries, which are superimposed clips for 20 minutes. Then blood flow through the vessels was restored to achieve reperfusion. To study the effects of early ischemia-reperfusion injury of the animals taken out of the experiment after 1 hour at the end of reperfusion period and deferred on ther 12th day. The brain was removed on cold,
using stereotaxic atlas coordinates cortex parietal was taken, frontal and temporal lobes of the brain hemispheres, fixed in 10% solution of Buena. The analysis of histological sections was performed in the digital image analysis VIDAS-386 (Kontron Elektronik, Germany) in the spectrum of luminescence in a fluorescent microscope AXIOSKOP (Zeiss, Germany).
According to the results of the experimental studies the dynamics of RNA content in the neurons and glial cells of different cortical lobes of the cerebral emispheres under conditions of carotid ischemia-reperfusion of the brain in rats both without diabetes mellitus and with it was examined. The glial cells were found to respond more significantly to 20 minute carotid ischemia with one hour reperfusion in the cortex of the temporal lobe by the change of RNA concentration, while in the cortex of the frontal and parietal lobes the nervous cells do this. On the 12th day of ischemic-reperfusion period RNA concentration in the parietal cortex is lowered in the neurons and comes back to the control readings in the glial cells, and it remains high in the glial and nervous cells of the frontal and temporal lobes. Diabetes mellitus causes reverse RNA reaction of the neurocytes in the parietal and frontal lobes in response to ischemic-reperfusion lesion of the brain in both periods of observation, eliminates RNA reaction of the glial cells in the parietal lobe in the early ischemi-reperfusion period; the cortex of the frontal lobe –
during both periods of observation; the cortex of the temporal lobe – on the 12th day in comparison with the same one in the control group of animals.
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Author Name: T. KMET
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Keywords: cerebral ischemia-reperfusion, diabetes mellitus, neurons, glia, RNA