A new colloid-hyperosmolar infusion solution HAES-LX-5% containing hydroxyethyl starch 130/0.4 (5%), xylitol (5%), sodium lactate (1.5%), electrolytes Na+, K+, Ca++, Mg++ and Cl- was developed. Osmolarity of this drug is 890 mOsm/l. The aim of experiment was to study the effect of intravenous HAES-LX-5% on the course of experimental toxic hepatitis.
Materials and methods. The experimental model of toxic hepatitis (TH) was created by subcutaneous injection of 50% oil solution of carbon tetrachloride (CCl4), which was administered daily for 6 days with the dose of 0,5 ml/kg. The animals from the first day of CCl4 administration for 14 days were treated intravenously with 0,9% NaCl or HAES-LX-5% - 2.5 ml/kg, once per day. The control group consisted of rabbits, which during administration of CCl4 infusion drugs were not applied. Biochemical and coagulation parameters in blood of animals were examined prior to the experiment, on day 7 and 15. At the end of the experiment changes in morphological picture of rabbit liver were studied.
In groups of animals who underwent infusion therapy with 0,9% NaCl and HAES-LX-5% on the day 7 of experiment decrease of intoxication was observed compared to the control group "TH without treatment," as evidenced by the much smaller increase of laboratory indicators alanine aminotransferase (ALT) (p<0.05), aspartate aminotransferase (p<0.05), lactate dehydrogenase, total bilirubin, prothrombin time, thrombin time, activated partial thromboplastin time.
Despite the fact that after 15 days in all three groups of animals laboratory parameters returned to the normal range, morphologically group of animals "TH untreated" showed a set of changes that are appropriate manifestations of toxic hepatitis, hepatic necrosis of centrolobular zone; hydropic degeneration of individual cells of intralobular zone. When conducting fluid therapy of toxic hepatitis with 0,9% NaCl solution or HAES-LX-5% morphologically pathological changes in liver were less pronounced. When comparing the effectiveness of various solutions for infusion therapy we found that the application of HAES-LX-5% resulted in significantly lower increase of ALT on the 7th day of experiment than in the "TH + 0,9% NaCl» group (p<0.05). The difference of other laboratory parameters in groups of 0,9% NaCl and HAES-LX-5% were not statistically significant.
On the 15th day of the experiment no dependence of laboratory parameters dynamics on application of 0.9% NaCl solution or drug HAES-LX-5% was recorded. At the same time, morphologically in the group "TH + 0,9% NaCl» degenerative changes of centrolobular zones of hepatocytes were observed; as well as slight lymphocytic infiltration of portal tracts. The animals of "TH + HAES-LX-5%" group only in two cases showed signs of toxic hepatitis and multiple sclerosis of small portal tracts. In other cases, hepatic lobules structures were intact. According to the results of the experiment it can be concluded that the drug HAES-LX-5% is safe, has no adverse effect on liver disease and the effectiveness of infusion therapy of experimental toxic hepatitis with this solution for some parameters predominates 0.9% solution of NaCl.
Keywords: hydroxyethyl starch, colloid hyperosmolar solutions, HAES-LX-5%, experimental toxic hepatitis.
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Author Name: ??????????? ?.?., ????? ?.?., ??????????? ?.?., ???????? ?.?., ????? ?.?., ???????? ?.?., ?????? ?.?.
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Keywords: hydroxyethyl starch, colloid hyperosmolar solutions, HAES-LX-5%, ex - perimental toxic hepatitis