The increasing demand for novel biocatalysts stimulates exploration of natural resources. Metagenomics, a culture independent approach, represent a sheer unlimited resource for discovery of novel biocatalysts from uncultured microorganisms. For industries that produce bulk commodities, a single enzyme backbone with superior functionality that has an entirely new sequence would be useful to avoid infringing competitor’s intellectual property rights. Two types of screening have been used to identify clones carrying desired traits from metagenomic libraries: function-based screening, and sequence-based screening. The main application depends on the construction of a metagenomic library in a suitable vector and host with subsequent high-throughput screening. This review focuses on the metagenomic approach for exploring novel enzyme discovery.
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Author Name: T. Choudhury and A.K. Pandey
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Keywords: Metagenomics, Novel biocatalyst, Hybridization, Phylogenic, Fungi
ISSN: 0972-7167
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