Background: Vast variety of intermediate factors including cell cycle regulators, growth factors, transcription
factors, and signaling pathways are involved in hematopoietic stem cell (HSC) commitment and differentiation
into distinct lineages. VHL, Ecad, and RUNX3 are among these. Epigenetics is currently introduced as a
potential mechanism to control the gene regulation. The aim of this study is to reveal the correlation between
the expression level and methylation pattern of mentioned genes after in vitro differentiation of cord blood
HSCs into erythroid lineage mediated by erythropoietin.
Materials and Methods: After isolation and expansion, the CD34+ cord blood stem cells were divided into
two parts. The first part was used to extract the DNA and RNA and the second to differentiate into erythroid
lineage. Methylation specific PCR (MSP) and Real-time PCR were used to determine the methylation status
and expression levels of the genes, respectively.
Results: Although the significant upregulation observed for VHL and Ecad genes and a down-regulation for
RUNX3 gene after differentiation, no remarkable changes were seen in methylation pattern compared with
cord blood HSCs by MSP technique.
Conclusion: It is appearing that methylation pattern in promoter region has not an effective role in expression
of VHL, Ecad, and RUNX3. Moreover, considering the inability of MSP method to detect subtle differences in
methylation level a more sensitive method is needed to distinguish the methylation levels of these genes before
and after erythroid differentiation
Real Time Impact Factor:
1.66667
Author Name: Mehdi Azad , Mehdi Goudarzi , Ali Dehghanifard , Mousa Vatanmakanian, Mehdi Sahmani
URL: View PDF
Keywords: Methylation, gene expression, erythropoietin, differentiation
ISSN: 2345-3346
EISSN:
EOI/DOI:
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