Phycocyanin is a major light harvesting pigment of cyanobacteria. Phycocyanin has gained importance in many biotechnological applications in food sciences, therapy, immunodiagnostics, cosmetics and pharmacological processes. Screening of cyanobacterial strain showed maximum phycobiliprotein in Anabaena NCCU-9. It was followed by optimization of extraction conditions. Sodium phosphate buffer, pH-7.5, supplemented with 0.15 M sodium chloride, freezing and thawing (-20 & 4°C) enhanced the total phycobiliprotein extraction. In this paper purification and characterization of phycocyanin: one of the important phycobiliprotein is being presented. Extraction, ammonium sulphate precipitation, dialysis, gel chromatography and SDS PAGE gave phycocyanin of 4.45 purity value and a band of 18.5 kDa.
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Author Name: Hemlata Gunjan Pandey, fareha Bano . Tasneem Fatma
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Keywords: Anabaena, Extraction, Optimization, Phycobiliprotein, Phycocyanin, Purity value
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EISSN: 2229 – 6905
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