It is now evident that plant tissue culture is an essential component of plant Biotechnology which offers novel approach to the production, propagation conservation and manipulation of plants. In Stevia rebaudiana Bertoni the use of in- vitro techniques as alternative or complement to conventional procedures of propagation and genetic improvement could facilitate obtaining disease free homogenous populations. The purpose of the present study was to show the effect of Cyanobacterial Media and Murashige and Skoog media for in-vitro propagation of Stevia rebaudiana Bertoni by taking different explants and by taking different concentrations of hormones. Using cyanobacterial media might be useful to obtain in-vitro callusing from the explants of Stevia rebaudiana Bertoni.
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Author Name: Tiwari Seema, Rashmi Arnold, Amit Tiwari, R. M. Mishra and U. K. Chauhan
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Keywords: Cyanobacterial Media (CM), Murashige and Skoog’s Medium (MS), Stevia rebaudiana Bertoni, in-vitro propagation.
EISSN: 2229 – 6905