In the present study Immunoglobulin G was purified from serum of Yak by gel filtration chromatography on Sephacryl S-200. SDS- PAGE of purified yak IgG showed four major polypeptides of 62.94 kDa, 53.29 kDa 29.22 and 28.21 kDa. The purified Immunoglobulin has been found to be immuno-reactive by DID test and Western Blot analysis when treated against hyper-immune sera.
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Author Name: Debjani Saha, Subhasis Batabyal, Saibal Chattopadhyay, Souvik Barui, Abhijit Barui
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Keywords: Yak, IgG, Purification, Characterization.
ISSN: 2277-470X
EISSN: 2319-247X
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