Interpenetrating polymer network (IPN) architectures have been conceived to improve the mechanical properties of a fibrin gel. Self-supported biomaterials are synthesized rapidly (one pot – one shot process) and combine the properties of both a protein gel and a synthetic polymer. IPN architectures have been characterized with biochemical (ELISA), chemical (solvent extraction) and physicochemical (rheology, DMA) methods. Mechanical properties of a fibrin gel were improved (viscoelasticity x 100) by associating it with a polymer network (PEO, PVA) inside IPN architecture. The network composition insures the material biodegradability through enzyme hydrolysis. These co-network IPNs are the first ones to be potentially biodegradable through tunable fragmentation, then elimination. They also exhibit the unique feature for a protein-based biomaterial of being non-retractable when used as support for fibroblast culture. The material is biocompatible as demonstrated with human dermal fibroblasts. Adhesion, viability and proliferation of human dermal fibroblasts have been measured for various IPN compositions with Live/dead test and by confocal microscopy. This innovative biomaterials present good potentiality as supports for skin construct.
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Author Name: Veronique Larreta-Garde, Marie Deneufchatel, Laurent Bidault, Mathilde Hindie, Cedric Vancaeyzeele, Odile Fichet
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Keywords: IPN, tissue engineering, biodegradability
ISSN: 2068-0783
EISSN: 2068-0783
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