Introduction: Breast cancer is progressively more global problem. It is considered the first or second most common cancer and major cause of death in women globally. Human epidermal growth factor receptor (HER-2) is proto-oncogene, a transmembrane glycoprotein receptor (p185) with tyrosine kinase activity. Mutation and overexpression of HER-2 gene leads to tumorigenesis through the overexpression of HER-2 enhances and prolongs the signals that trigger the transformation of the cells. Objective: we aimed to study and investigate the association of HER-2 (ILe655Val: rs 1136201) gene polymorphism with the risk of breast cancer. Methods: The genotypes and allele frequencies of HER-2 (ILe655Val) gene polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in 300 breast cancer patients and 200 controls after extraction of DNA from blood samples of all participants. Results: The results revealed that the HER-2 gene A?G (ILe 655 Val) SNP genotype frequencies of wild genotype AA, heterozygous genotype AG and homozygous genotype GG were 47 %, 41.7 % and 11.3 % in cases of breast cancer group and 65 %, 29 % and 6% in the control group respectively. The heterozygous genotype AG was found increased the risk of breast cancer by about two folds higher than those of the wild genotype AA (OR= 1.62, 95% C.I= 1.15–2.4, P = 0.022). In contrast, the homozygous genotype GG was significantly increased the risk of breast cancer by about two and half folds higher than that found in wild genotype AA (OR= 2.44, 95% C.I= 1.23–5.1, P = 0.014). Conclusion: We concluded that the Val allele of HER-2 gene was increased the risk of breast cancer in patients of Iraqi population.
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Author Name: Anwar M. Al-Janabi. Abdul Hussein Alwan Algenabi. Talib Hussein Kamoona. Salih M. Alkhafaji.
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Keywords: Breast cancer , HER-2 , Polymorphism , SNP , Iraq
ISSN: 2320-5407
EISSN: 2320-5407
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