Bacterial degradation trial for diazinon residues was performed using bacterial strains labeled as S1, S2, S3, S4 and S5 isolated from newly reclaimed agricultural soil. Diazinon residues were determined at successive intervals untill 30 days after incubation in liquid medium containing bacteria paralleled with control samples. Diazinon recovery rate was carried out at 0.1 and 1 mg kg-1 levels; values were 95.77 and 101.12%, respectively, limit of detection (LOD) was 0.01 mg kg-1, while limit of quantification (LOQ) was 0.03 mg kg-1. Diazinon half-life values (RL50) were 9.36, 8.57, 12.71, 7.51 and 8.20 days for S1, S2, S3, S4 and S5, respectively, while control value was 14.06 days. No significant effect on diazinon occurred with S3 (Staphylococcus sciuri) treatment, while S1 (Pseudomonas aeruginosa), S2 (Bacillus amyloliquefaciens), S4 (Bacillus pseudomycoides) and S5 (Bacillus licheniformis) treatments showed significant effect, that increased diazinon degradation rate compared to control treatment. Only one degradation product was detected from bacterial degradation namely 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP).
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Author Name: Tamer Mohamed Ahmed Mohamed Thabit and Medhat Ahmed Hassan El-Naggar
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Keywords: Insecticides, biodegradation, bacteria, agricultural soil, degradation products
ISSN: 2075-1141
EISSN: 2074-9546
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